Cytoskeletal Alteration Is an Early Cellular Response in Pulmonary Epithelium Infected with Aspergillus fumigatus Rather than Scedosporium apiospermum

摘要: Invasive aspergillosis and scedosporiosis are life-threatening fungal infections with similar clinical manifestations in immunocompromised patients. Contrarily, Scedosporium apiospermum is susceptible to some azole derivative but often resistant amphotericin B. Histopathological examination alone cannot diagnose these two species. Pathogenesis studies could contribute explore candidate protein markers for new diagnosis treatment methods leading a decrease mortality. In the present study, proteomics was conducted identify significantly altered proteins A549 cells infected or without Aspergillus fumigatus S. as measured at initial invasion. Protein validation performed immunogold labelling alongside immunohistochemical techniques lungs from murine models. Further, cytokine production measured, using Bio-Plex-Multiplex immunoassay. The cytoskeletal HSPA9, PA2G4, VAT1, PSMA2, PEX1, PTGES3, KRT1, KRT9, CLIP1 CLEC20A were mainly changed during A. infection, while immunologically activated WNT7A, GAPDH ANXA2 principally infection. These involved internalisation structural destruction pulmonary disorders. Interleukin (IL)-21, IL-1α, IL-22, IL-2, IL-8, IL-12, IL-17A, interferon-γ tumour necrosis factor-α upregulated both scedosporiosis, although more predominately latter, accordance chitin synthase-1 matrix metalloproteinase levels. Our results demonstrated that invasion, primarily host cellular integrity, whereas chiefly induced extensively modulated immune responses.