Cloning and analysis of Candida cylindracea lipase sequences.
作者： Marina Lotti , Rita Grandori , Fabrizia Fusetti , Sonia Longhi , Stefania Brocca
DOI: 10.1016/0378-1119(93)90760-Z
关键词:
摘要: Lipases (Lip) hydrolyze triglycerides into fatty acids and glycerol. Lip produced by the yeast Candida cylindracea are encoded multiple genomic sequences. We report molecular cloning characterization of three genes from this family. They encode putative mature 57-kDa proteins 534 amino (aa). To date, five Lip-encoding sequences C. have been characterized in our laboratory. The deduced aa share an overall homology 80%. These aligned with each other those homologous enzymes, mould Geotrichum candidum acetylcholinesterase Torpedo californica, whose three-dimensional structures solved X-ray analysis. appear to a structural organization similar that described for both enzymes.
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