High-resolution 3D CANCA NMR experiments for complete mainchain assignments using Cα direct-detection

摘要: The primary limitation of solution state NMR with larger, highly dynamic, or paramagnetic systems originates from signal losses due to fast transverse relaxation. This is related the high gyromagnetic ratio γ protons, which are usually detected. Thus, it attractive consider detection nuclei lower γ, such as 13C, for extending size limits NMR. Here, we present an approach complete assignment Cα and N resonances in relaxing proteins using a detected 3D CANCA experiment perdeuterated proteins. correlates alpha carbons sequentially adjacent succeeding nitrogen carbons. enables elongation chain assigned residues simply by navigating along both carbon dimensions “stairway” procedure. simultaneous use sequential connectivities makes more robust than conventional experiments, rely solely on single 13C indirect dimension sequenti...