Hematogones: a multiparameter analysis of bone marrow precursor cells

摘要: Morphologically distinct lymphoid cells with homogeneous, condensed chromatin and scant cytoplasm can be observed in large numbers the bone marrow of children a variety hematologic nonhematologic disorders. In some patients, these may account for greater than 50% cells, creating picture that confused acute lymphoblastic leukemia (ALL) or metastatic tumor. Although originally called hematogones (HGs), other names have been proposed unique cells. The clinical significance expanded HGs has not resolved, biologic features are incompletely described. this study, we correlate clinical, morphologic, cytochemical, flow cytometric, molecular, cytogenetic properties samples from 12 substantial (range 8% to 55% cells). Diagnoses patients included anemia, four; neutropenia, one; anemia idiopathic thrombocytopenic purpura, two; retinoblastoma, Ewing's sarcoma, germ cell tumor, one. Flow cytometric analyses demonstrated spectrum extending early B-cell precursors (CD10+, CD19+, TdT+, HLA-Dr+) mature surface immunoglobulin-bearing B corroborating our morphologic impression HGs, intermediate forms, lymphocytes. DNA content was normal, no clonal abnormality identified by either immunoglobulin T-cell receptor (TCR) gene rearrangement studies. Follow-up ranged 3 months years. None developed involvement solid possible role immune recovery hematopoiesis is presented.