Methods of commercial production and extraction of protein from seed
作者： John A. Howard
DOI:
关键词:
摘要: A method for extraction of heterologous protein from plant seed comprises extracting the germ portion and purifying germ. Enhanced expression in is provided, allows improved efficiency production, cost savings. Directing to further increases levels protein. The ubiquitin promoter preferentially directs seed.
lens.org参考文章(22)
David L. Banaszak, Remote control structural exciter ,(1997)
Elizabeth E. Hood, Derrick R. Witcher, Sheila Maddock, Terry Meyer, Chris Baszczynski, Michele Bailey, Pam Flynn, James Register, Lisa Marshall, Diane Bond, Ellen Kulisek, Ann Kusnadi, Roque Evangelista, Zivko Nikolov, Cynthia Wooge, Richard J. Mehigh, Ronald Hernan, William K. Kappel, David Ritland, Chun Ping Li, John A. Howard, Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification Molecular Breeding. ,vol. 3, pp. 291- 306 ,(1997) , 10.1023/A:1009676322162
John A. Howard, Benjamin P. All, Expression cassettes and methods for delivery of animal vaccines ,(1996)
Marc C. Albertsen, Sheila Maddock, John A. Howard, Induction of male sterility in plants by expression of high levels of avidin ,(1998)
S�nke Holtorf, Klaus Apel, Holger Bohlmann, COMPARISON OF DIFFERENT CONSTITUTIVE AND INDUCIBLE PROMOTERS FOR THE OVEREXPRESSION OF TRANSGENES IN ARABIDOPSIS THALIANA Plant Molecular Biology. ,vol. 29, pp. 637- 646 ,(1995) , 10.1007/BF00041155
Joel Vandekerckhove, Jozef Van Damme, Mieke Van Lijsebettens, Johan Botterman, Marc De Block, Martine Vandewiele, Ann De Clercq, Jan Leemans, Marc Van Montagu, Enno Krebbers, Enkephalins Prouduced in Transgenic Plants Using Modified 2S Seed Storage Proteins Nature Biotechnology. ,vol. 7, pp. 929- 932 ,(1989) , 10.1038/NBT0989-929
A.R. Kusnadi, E.E. Hood, D.R. Witcher, J.A. Howard, Z.L. Nikolov, Production and purification of two recombinant proteins from transgenic corn. Biotechnology Progress. ,vol. 14, pp. 149- 155 ,(1998) , 10.1021/BP970138U
Ulrike Fiedler, Udo Conrad, High-level production and long-term storage of engineered antibodies in transgenic tobacco seeds Nature Biotechnology. ,vol. 13, pp. 1090- 1093 ,(1995) , 10.1038/NBT1095-1090
Helmut Bäumlein, Wout Boerjan, Istvan Nagy, Reinhard Panitz, Dirk Inzé, Ulrich Wobus, Upstream sequences regulating legumin gene expression in heterologous transgenic plants Molecular Genetics and Genomics. ,vol. 225, pp. 121- 128 ,(1991) , 10.1007/BF00282650
P. Gallusci, F. Salamini, R. D. Thompson, Differences in cell type-specific expression of the gene Opaque 2 in maize and transgenic tobacco. Molecular Genetics and Genomics. ,vol. 244, pp. 391- 400 ,(1994) , 10.1007/BF00286691