Electrospray induced surface activation of polystyrene microbeads for diagnostic applications

摘要: Electrospray is generally regarded as a “soft” technique due to the absence of any observable molecular fragmentation or destruction. This study reports on novel and easy way induce surface activation polystyrene microbeads through electrospray deposition into grounded aqueous electrolyte solution bath. process, nicknamed EISA, which stands for induced activation, proposes that when highly charged microbead formed by process sinks solution, it behaves like spherical capacitor discharges in conductive liquid. The energy released leads breakup bonds water oxidation with oxygen. Further reactions produce carboxylated was confirmed X-ray photoelectron spectroscopy (XPS) protein coupling. An immunoassay based these modified also developed presented use syphilis detection, demonstrating reliable signal-to-noise ratio between positive negative results.