A detailed procedure for CRISPR/Cas9-mediated gene editing in tilapia
作者： Minghui Li , Shengfei Dai , Xingyong Liu , Hesheng Xiao , Deshou Wang
DOI: 10.1007/S10750-020-04414-8
关键词:
摘要: Reverse genetics approaches are critical for uncovering complex biological processes and genetic engineering. Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) system (CRISPR/Cas9) has been widely used generating mutants in a large number of species. The Nile tilapia (Oreochromis niloticus), gonochoristic teleost with XX/XY sex determination system, is good model studying fish reproduction. Here, we describe detailed procedure gene mutation by CRISPR/Cas9. This protocol covers selection target sites, vitro RNA transcription, artificial insemination microinjection one cell stage embryos. We also provide details detection somatic germline transmitted mutations, fast establishment mutant lines discuss some practical advices. will facilitate broader applications CRISPR/Cas9 studies as well other aquaculture fishes.
springer.com
sci-hub.st 参考文章(52)
Dmitry Y. Guschin, Adam J. Waite, George E. Katibah, Jeffrey C. Miller, Michael C. Holmes, Edward J. Rebar, A rapid and general assay for monitoring endogenous gene modification. Methods of Molecular Biology. ,vol. 649, pp. 247- 256 ,(2010) , 10.1007/978-1-60761-753-2_15
Andrea Rossi, Zacharias Kontarakis, Claudia Gerri, Hendrik Nolte, Soraya Hölper, Marcus Krüger, Didier Y. R. Stainier, Genetic compensation induced by deleterious mutations but not gene knockdowns Nature. ,vol. 524, pp. 230- 233 ,(2015) , 10.1038/NATURE14580
Woong Y Hwang, Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander, Randall T Peterson, J-R Joanna Yeh, J Keith Joung, Efficient genome editing in zebrafish using a CRISPR-Cas system Nature Biotechnology. ,vol. 31, pp. 227- 229 ,(2013) , 10.1038/NBT.2501
Yuyu Niu, Bin Shen, Yiqiang Cui, Yongchang Chen, Jianying Wang, Lei Wang, Yu Kang, Xiaoyang Zhao, Wei Si, Wei Li, Andy Peng Xiang, Jiankui Zhou, Xuejiang Guo, Ye Bi, Chenyang Si, Bian Hu, Guoying Dong, Hong Wang, Zuomin Zhou, Tianqing Li, Tao Tan, Xiuqiong Pu, Fang Wang, Shaohui Ji, Qi Zhou, Xingxu Huang, Weizhi Ji, Jiahao Sha, Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos Cell. ,vol. 156, pp. 836- 843 ,(2014) , 10.1016/J.CELL.2014.01.027
Yun-Lv Sun, Dong-Neng Jiang, Sheng Zeng, Chong-Jiang Hu, Kai Ye, Chao Yang, Shi-Jie Yang, Ming-Hui Li, De-Shou Wang, Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Nile tilapia (Oreochromis niloticus) Aquaculture. ,vol. 433, pp. 19- 27 ,(2014) , 10.1016/J.AQUACULTURE.2014.05.035
Andreas R. Dunz, Ulrich K. Schliewen, Molecular phylogeny and revised classification of the haplotilapiine cichlid fishes formerly referred to as "Tilapia". Molecular Phylogenetics and Evolution. ,vol. 68, pp. 64- 80 ,(2013) , 10.1016/J.YMPEV.2013.03.015
Nannan Chang, Changhong Sun, Lu Gao, Dan Zhu, Xiufei Xu, Xiaojun Zhu, Jing-Wei Xiong, Jianzhong Jeff Xi, Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos Cell Research. ,vol. 23, pp. 465- 472 ,(2013) , 10.1038/CR.2013.45
Wei Li, Fei Teng, Tianda Li, Qi Zhou, Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems Nature Biotechnology. ,vol. 31, pp. 684- 686 ,(2013) , 10.1038/NBT.2652
Timothy R. Sampson, Sunil D. Saroj, Anna C. Llewellyn, Yih-Ling Tzeng, David S. Weiss, A CRISPR/Cas system mediates bacterial innate immune evasion and virulence Nature. ,vol. 497, pp. 254- 257 ,(2013) , 10.1038/NATURE12048
Ming-Hui Li, Hui-Hui Yang, Meng-Ru Li, Yun-Lv Sun, Xiao-Long Jiang, Qing-Ping Xie, Ting-Ru Wang, Hong-Juan Shi, Li-Na Sun, Lin-Yan Zhou, De-Shou Wang, Antagonistic roles of Dmrt1 and Foxl2 in sex differentiation via estrogen production in tilapia as demonstrated by TALENs. Endocrinology. ,vol. 154, pp. 4814- 4825 ,(2013) , 10.1210/EN.2013-1451