An improved procedure for production of transgenic flax plants using Agrobacterium tumefaciens

摘要: Abstract A procedure for increased efficiency of production transgenic flax plants was developed by increasing in the cellular transformation intensity on inoculated hypocotyls, judicious choice selection agent and optimization scheme. The binary plasmid p3SSGUSINT octopine-type Agrobacterium tumefaciens strain GV2260 used as vector system. An intron-containing β-glucuronidase (GUS) gene driven CaMV 35S promoter served reported histochemical assay. neomycin phosphotransferase II (NPT II) T-DNA provide resistance to kanamycin or geneticin (G418) selectable market. Cellular greatly improved preculture partial removal epidermal cells prolonged cocultivation duration with Agrobacterium. When hypocotyls initiated shoot induction medium, provided a more efficient transformed shoots than G418. Application strong pressure (100 200 mg/l kanamycin) resulted an frequency (11.7% 16.4%, respectively) also reduced occurrence ‘escapes’. increase actual proportion explants eventually led shoots. With optimized described here, approximately 13% produced existence transgenes confirmed polymerase chain reaction.