Biofunctionalized Magnetic Nanoparticles for Specifically Detecting Biomarkers of Alzheimer’s Disease in Vitro
作者： Ming-Jang Chiu , Che-Chuan Yang , Shieh-Yueh Yang , Jen-Jie Chieh , Herng-Er Horng
DOI: 10.1021/CN200028J
关键词:
摘要: Magnetic nanoparticles biofunctionalized with antibodies against β-amyloid-40 (Aβ-40) and Aβ-42, which are promising biomarkers related to Alzheimer’s disease (AD), were synthesized. We characterized the size distribution, saturated magnetizations, stability of magnetic conjugated anti-Aβ antibody. In combination immunomagnetic reduction technology, it is demonstrated such able label Aβs specifically. The ultralow-detection limits assaying in vitro using via determined a concentration ∼10 ppt (10 pg/mL). Further, signals Aβ-40 Aβ-42 human plasma from normal samples AD patients analyzed, results showed significant difference between these two groups. These show feasibility as reagents for low-concentration through reduction, an...
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