Specific expression of matrix metalloproteinases 1, 3, 9 and 13 associated with invasiveness of breast cancer cells in vitro.

摘要: Several matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) were studied in highly invasive (MDA-MB-231) slightly (MCF-7, T47D, BT-20) breast cancer cell lines. Investigations carried out at the protein level and/or mRNA level, either cells cultured as monolayers on plastic, or seeded a thin layer Matrigel basement membrane matrix. Analysis MMP expression by RT-PCR showed MMP-1, MMP-3, MMP-13 MDA-MB-231 cells, but not The extracellular secretion MMP-1 MMP-3 MDA-MB 231 could be also shown ELISA. TIMP-1 TIMP-2 mRNAs found all lines, however, both TIMPs was much higher than other When matrix, MMP-9 induced only, assessed zymography experiments. potential evaluated vitro through significantly inhibited inhibitor BB-2516, 25% 50% concentrations 2 × 10−6M 10−5M, respectively. In conclusion, our data show that MCF-7 BT-20 express MMP-13. which is specifically up-regulated contact to Matrigel, may play key role invasiveness membranes.