Interstitial telomeres are hotspots for illegitimate recombination with DNA molecules injected into the macronucleus of Paramecium primaurelia.

摘要: DNA molecules injected into the macronucleus of Paramecium primaurelia replicate either as free linear telomerized or chromosome integrated molecules. In present study we show that when a 1.77 kb BamHI fragment harbouring his3 gene Saccharomyces cerevisiae was microinjected macronucleus, fraction are via an illegitimate recombination process. The were mostly inserted at their extremities multiple points in genome by replacing sequences. However, insertion sites not totally random. Roughly 30% next to telomeric repeats. These repeats chromosomes but occupy internal interstitial position. We argue such hotspots for integration probability random near site, which there 25-60 is between 5 x 10(-4) and 3 10(-5).