A rapid and quantitative method for the isolation of gangliosides and neutral glycosphingolipids by DEAE-silica gel chromatography.
作者： S K Kundu , S K Roy
DOI: 10.1016/S0022-2275(20)41313-6
关键词:
摘要: DEAE-silica gel has been shown to be an improvement over DEAE-Sephadex for the quantitative isolation of gangliosides and neutral glycosphingolipids from animal tissues or cells. Preliminary results indicated that it can also used protein separation. Direct comparative studies with showed preferences former following reasons: i) faster flow rate; ii) more rapid equilibration starting buffer; iii) easier regeneration; iv) economical; v) a lesser susceptibility microbial attack.
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