Response of glandular versus basal rat ventral prostatic epithelial cells to androgen withdrawal and replacement.

摘要: Light microscope autoradiography was utilized to investigate the separate proliferative activities of basal and glandular cell types during androgen-mediated restoration ventral prostatic epithelium in castrated rats. Our objective determine whether population is restricted subtype under these experimental conditions. In intact animal, substantial numbers as well cells were found be proliferating. The labeling index (LI) 3.75% +/- 0.3% or 1.85 0.4 x 10(5) labeled per prostate. For comparable figures 0.38% 3.32 1.5 10(5). Seven days after surgical castration, both subtypes persisted. However, only significantly reduced (66% decrease). When androgens replaced by daily subcutaneous injection (testosterone propionate, 2 mg/0.2 ml sesame oil), responded with increased proliferation. temporal pattern response displayed each type very similar. A greater proportion than responded, ie, 28% vs 11% LI. terms approximate peak response, value for larger, 2.4 1.1 10(6) cells. These data clearly indicate that normal conditions testosterone stimulation, are not sole proliferating epithelium. Thus, cells, a distinct morphologic population, homogeneously undifferentiated stem which give rise differentiated nonproliferating epithelial Aspects can nevertheless interpreted support idea maintained single lineage system comprise subpopulation Alternatively, two populations may no presented unequivocally links precursor-progeny relationship.