Enzymatic release of germ tube-specific antigens from cell walls of Candida albicans.

摘要: Abstract The differences between blastospores and germ tubes of Candida albicans, as previously shown by immunofluorescence, were further studied comparing digests cell walls both growth forms. Organisms surface labeled with 125I, digested enzymatically. When Zymolase treated polyclonal, polyspecific antiserum to C. albicans 441B, which stains only in immunofluorescence assays, components molecular weights 200,000 155,000 immunoprecipitated from strain B311, but nothing was recovered blastospores. Whereas the 200,000-molecular-weight component found three strains tested, 155,000-molecular-weight B311. unadsorbed antiserum, an additional precipitated forms a weight greater than marker. All antigens mannoproteins, their abilities bind concanavalin A be 125I. Also, all located on surface, following criteria: adsorption antisera live organisms removed antibody these components, eluted surfaces whole components. Components common forms, well tube-specific detected trypsin chymotrypsin digests, differed those digests. Thus, determinants enzymatically released wall