Complex formation with vesicular stomatitis virus phosphoprotein NS prevents binding of nucleocapsid protein N to nonspecific RNA.

摘要: The interactions between the nucleocapsid protein N and either RNA or phosphoprotein NS of vesicular stomatitis virus (VSV) were studied by transcription mRNAs from SP6 vectors, followed translation in a rabbit reticulocyte lysate. Nascent bound tightly to added labeled RNA, as well endogenous This binding was demonstrated three independent techniques. First, migrated identically series sharp, closely spaced bands nondenaturing gel system. Second, translated behaved stable ribonucleoprotein complex CsCl gradients sedimented same density authentic N-RNA template VSV. Third, protected fragments digestion RNase A. None RNA-binding criteria satisfied two deletion mutants other components evidence suggests that observed nascent not sequence specific, contrast encapsidation process during VSV replication. Moreover, prior formation N-NS complexes totally abolished N. Thus, we propose may be responsible for conferring specificity occurs genome