作者: Abbas Hawwari , Michael S. Krangel
DOI: 10.1084/JEM.20050680
关键词:
摘要: Murine Tcrd and Tcra gene segments reside in a single genetic locus undergo recombination CD4 − CD8 (double negative [DN]) + positive [DP]) thymocytes, respectively. TcraTcrd variable are subject to complex regulation. Only small subset of ∼100 contributes substantially the adult TCR δ repertoire. Moreover, although most contribute α repertoire, that J proximal preferentially used during primary recombination. We investigate role local chromatin accessibility determining developmental pattern segment find be heterogeneous with respect acetylation histones H3 H4. Those dominate repertoire hyperacetylated DN independent their position locus. show dramatic increases histone germline transcription DP result super long-distance regulation by enhancer. Our results imply differences biases thymocytes extend distance over which enhancer can regulate structure remarkable 525 kb.