Molecularly bonded chitosan prepared as chiral stationary phases in open-tubular capillary electrochromatography: comparison with chitosan nanoparticles bonded to the polyacrylamide phase.

摘要: Abstract The chiral selector, chitosan (CS), was attached to the silanized capillary via a silane coupling agent, (3-glycidyloxypropyl)trimethoxysilane (GTS), form GTS–CS capillary, and results for this were compared with those of previous study on copolymerization CS methacrylamide (MAA) (forming MAA–CS capillary). did not exhibit enantioselectivity d / l -tryptophan, whereas GTS–BSA which prepared by replacement bovine serum albumin (BSA), succeeded in separation an Rs = 2.4 Tris buffer (50 mM, pH 8.5). To increase attachment, units crosslinked succinic acid, resulting GTS–CS–s phase improved resolution 1.9. Alternatively, SiH–CS–s constructed attachment silicon hydride stepwise silanization hydrosilation reactions crosslinking but approach could only achieve 1.4 9.5). Although capillaries still inferior (Rs = 3.8), enantioselectivities three all range 1.4–1.6. For (±)-catechin sample, plate heights conditioned 8.5 60% MeOH modifier 0.9 cm ((−)-catechin) 6.0 cm ((+)-catechin)) 2.9 cm (−) 3.2 cm (+), respectively, these comparable (2.5 cm (−), (+)) 6.6 phosphate 80% MeOH. Finally, racemate ibuprofen, weakly acidic anti-inflammatory drug, successfully baseline resolved borate buffers, 30 mM at 7.5 10 mM 8.0, respectively.