Enhanced photodynamic efficacy towards melanoma cells by encapsulation of Pc4 in silica nanoparticles.

摘要: Nanoparticles have been explored recently as an efficient means of delivering photosensitizers for cancer diagnosis and photodynamic therapy (PDT). Silicon phthalocyanine 4 (Pc4) is currently being clinically tested a photosensitizer PDT. Unfortunately, Pc4 aggregates in aqueous solutions, which dramatically reduces its PDT efficacy therefore limits clinical application. We encapsulated using silica nanoparticles (Pc4SNP), not only improved the solubility, stability, delivery drug but also increased compared to free molecules. Pc4SNP generated photo-induced singlet oxygen more efficiently than measured by chemical probe EPR trapping techniques. Transmission electron microscopy dynamic light scattering measurements showed that size particles range 25-30 nm. Cell viability demonstrated was phototoxic A375 or B16-F10 melanoma cells Pc4. photodamaged primarily through apoptosis. Irradiation presence resulted significant increase intracellular protein-derived peroxides, suggesting Type II (singlet oxygen) mechanism phototoxicity. More localized mitochondria lysosomes. Our results show these stable, monodispersed may be effective new formulation preclinical studies. expect modifying surface silicon encapsulating with antibodies specific will lead even better early targeted treatment future.