RNA Interference Protects Against 5-Lipoxygenase-Induced Cocarcinogen, Benzidine, Oxidation and Cytotoxicity in Human Tracheobronchial Epithelial Cells
作者： Yun Huang , Qingping Tan , Yue Wu , Hongxiang Zhu , Minru Xiong
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摘要: Lipoxygenase (LOX)-catalyzed cooxidation of the human carcinogen benzidine (BZD) has been shown in vitro enzyme systems. This study aimed to determine whether BZD could be activated by arachidonate 5-lipoxygenase (ALOX5) tracheobronchial epithelial cells (HBECs) using RNA interference strategy and a 5-LOX-specific inhibitor, AA861. We show that soybean LOX catalyzed BZD, generating diimine. Benzidine induced expression ALOX5 messenger 5-LOX protein HBECs, significantly decreased cell proliferation, but enhanced DNA damage apoptosis HBECs which were inhibited lentiviral-mediated small hairpin RNA-knockdown Thus, upregulate while inhibition or gene activity prevent BZD-induced cytotoxicity might caused 5-LOX-catalyzed oxidative activation BZD.
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