The nuclear status of human sperm cells by TEM image cytometry: nuclear shape and chromatin texture in semen samples from fertile and infertile men.

摘要: Changes in nuclear size, shape, and chromatin texture during spermiogenesis epididymal transport of human sperm were recently analyzed using transmission electron microscopy (TEM) image cytometry followed by multivariate statistical analysis data. In the present study, this same methodology was used to investigate morphology spermatozoa semen samples from fertile infertile men. Analysis carried out on a large series micrographs sections nuclei donor group with proven fertility patient mean infertility duration 10 years no obvious male or female factors (only slight decrease proportion heads normal noted routine tests). For group, it found that had significantly less flattened shape (i.e., increased roundness as consequence thickness decreased length). Furthermore, significant differences between groups for most parameters texture. condensed, there more homogeneous distribution different degrees condensation. addition, organization condensation along major axis nucleus be two groups. Stepwise linear discriminant indicated good classification rate only 66% nuclel patients when eight parameters, thus indicating striking heterogeneity both However, parameter ranking showed predominant discriminatory capacity compared size reinforcing need including tests evaluating status cells analysis.