Production of toxic shock syndrome toxin 1 by Staphylococcus aureus requires both oxygen and carbon dioxide.

摘要: The effect of O2 and CO2 on expression toxic shock syndrome toxin 1 (TSST-1) by Staphylococcus aureus was investigated under controlled growth conditions with continuous-culture techniques. To stimulate TSST-1 production, air anaerobic gas were premixed before delivery to the culture vessel. At a rate—or mass doubling time (td)—of 3 h, production specific (expressed as micrograms per milligram cell dry weight) 5.9-fold greater at an concentration 4% than conditions. Increasing 11% did not result in significant increase (P > 0.05) rate over that during but (4.9-fold; P < 0.001) growth. td 9 addition 3.5% resulted 7.6-fold production. When room sparged through growing increased significantly (by 3.4-fold) environment O2–remainder N2 studied, no observed; this also case 8% gas-flow rates 0.1, 0.2, 0.4 liters/min. In all experiments, biomass milligrams weight milliliter) increased, indicating metabolized S. aureus. Addition mix (4% O2, 10% CO2, 86% N2) 5.1- 6.8-fold 3.6-fold N2. agr mutant strain tested produced 6.1-fold more O2–10% CO2–86% These data suggest system, alone does trigger TSST-1; rather, both are required.