Mechanical spectroscopy of retina explants at the protein level employing nanostructured scaffolds.

摘要: Development of neuronal tissue, such as folding the brain, and formation fovea centralis in human retina are intimately connected with mechanical properties underlying cells extracellular matrix. In particular for tissue complex vertebrate retina, still a matter debate due to their relation numerous diseases well surgery, where tension can result detachment during cutting. However, measuring elasticity adult wholemounts is difficult until now only at surface have been characterized micrometer resolution. Many processes, however, pathological changes prone cause rupture detachment, respectively, reflected variations smaller length scales protein level. present work we demonstrate that freely oscillating cantilevers composed nanostructured TiO2 scaffolds be employed study frequency-dependent response mammalian explants nanoscale. Constituting highly versatile strong attachment long-term organotypic culture atop, these perform damped vibrations fingerprints derived using finite element calculations. Since adheres nanostructures via constitutive proteins on photoreceptor side latter stretched compressed vibration scaffold. Probing individual within proposed spectroscopy approach opens way studying mechanics, effect drugs