Detection of Pathogenic Organisms with Multicolor Molecular Beacons

摘要: The chapter describes a detection method that is based on the sequence-dependent hybridization of fluorogenic reporter molecules called “molecular beacons’’ and its applications. Molecular beacon probes represent new class oligonucleotides can hybridize report presence specific nucleic acids in homogeneous solutions. In addition, improvisations basic theme molecular beacons have also appeared literature. are primarily employed as highly amplicon homogeneous, real-time, multiplex gene amplification assays. On other hand, generation all five fluorescent colors during PCR indicates mycobacteria sample rifampin susceptible. Infection by certain types human papillomavirus (HPV) lead to cervical cancer; therefore, accurate identification these oncogenic HPV genotypes critical. One assay detects HPV-DNA SYBR Green® then distinguishes seven most prevalent high-risk using real-time PCR. They be used probes, not only for monitoring DNA vitro but distribution transport mRNAs living cells. current available applications pathogenic microorganisms listed chapter. Efforts being made explore their many areas including genotyping infectious agents mutation analysis drug-resistant pathogens.