PAPERCLIP Identifies MicroRNA Targets and a Role of CstF64/64tau in Promoting Non-canonical poly(A) Site Usage
作者： Hun-Way Hwang , Christopher Y. Park , Hani Goodarzi , John J. Fak , Aldo Mele
DOI: 10.1016/J.CELREP.2016.03.023
关键词:
摘要: Accurate and precise annotation of 3' UTRs is critical for understanding how mRNAs are regulated by microRNAs (miRNAs) RNA-binding proteins (RBPs). Here, we describe a method, poly(A) binding protein-mediated mRNA end retrieval crosslinking immunoprecipitation (PAPERCLIP), that shows high specificity ends compares favorably with existing mapping methods. PAPERCLIP uncovers previously unrecognized role CstF64/64tau in promoting the usage selected group non-canonical sites, majority which contain downstream GUKKU motif. Furthermore, mouse brain, discovers extended UTR sequences harboring functional miRNA sites reveals developmentally APA shifts, including one Atp2b2 evolutionarily conserved humans results gain site miR-137. provides powerful tool to decipher post-transcriptional regulation through vivo.
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