Screening of marine bacteria with bacteriocin-like activities and probiotic potential for ornate spiny lobster (Panulirus ornatus) juveniles.

摘要: Bacteriocins are ribosomally synthesized antimicrobial peptides, which have been found in diverse bacterial species of terrestrial origins and some from the sea. New bacteriocins with new characteristics, applications likely still awaiting discovery. The present study screened bacteria isolated marine animals interest to aquaculture industry for bacteriocin-like activities order uncover biodiversity bacteriocin producers, explore potential application aquaculture. In total, 24 100 isolates showed 7 these exerted activities. Sequencing 16S rRNA genes identified as members six genera Proteus, Providencia, Klebsiella, Alcaligenes, Bacillus Enterococcus. cases, further analysis housekeeping genes, rpoB Proteus recA well biochemical tests was necessary identification level, may represent novel species. seven bacteriocinogenic a wide spectrum against foodborne animal pathogens, opens way their use drugs probiotics food, aquaculture, livestock clinical settings. As case study, protective effect shortlisted were tested aquaculture-raised spiny lobster (Panulirus ornatus) juveniles. A single-strain (Bacillus pumilus B3.10.2B) three-strain (B. B3.10.2B, cereus D9, Lactobacillus plantarum T13) probiotic preparation added feed Panulirus ornatus juveniles, subsequently challenged pathogen Vibrio owensii DY05. Juveniles treatments displayed increased growth reduced conversion rates after 60 days, survival rate challenge relative control. This represents first evidence production by associated lobster, tiger shrimp, snubnose pompano cobia description V. owensii P. ornatus