Fucoidan Characterization: Determination of Purity and Physicochemical and Chemical Properties.

作者: Ahmed Zayed , Mona El-Aasr , Abdel-Rahim S. Ibrahim , Roland Ulber

DOI: 10.3390/MD18110571

关键词:

摘要: Fucoidans are marine sulfated biopolysaccharides that have heterogenous and complicated chemical structures. Various sugar monomers, glycosidic linkages, molecular masses, branching sites, sulfate ester pattern content involved within their backbones. Additionally, sources, downstream processes, geographical seasonal factors show potential effects on fucoidan structural characteristics. These characteristics documented to be highly related activities. Therefore, numerous qualitative quantitative determinations elucidation methods conducted characterize fucoidans regarding physicochemical features. Characterization of polymers is considered a bottleneck for further biological industrial applications. Consequently, the obtained results may different activities, which could improved afterward by functional modifications. The current article highlights spectrometric nonspectrometric applied characterization native fucoidans, including degree purity, monomeric composition, sulfation content, mass, linkages.

参考文章(208)
Jean-Pierre Hallé, Danielle Landry, Alain Fournier, Michèle Beaudry, Francois A. Leblond, Method for the quantification of alginate in microcapsules. Cell Transplantation. ,vol. 2, pp. 429- 436 ,(1993) , 10.1177/096368979300200511
I. Yu. Bakunina, O. I. Nedashkovskaya, S. A. Alekseeva, E. P. Ivanova, L. A. Romanenko, N. M. Gorshkova, V. V. Isakov, T. N. Zvyagintseva, V. V. Mikhailov, Degradation of fucoidan by the marine proteobacterium Pseudoalteromonas citrea Microbiology. ,vol. 71, pp. 41- 47 ,(2002) , 10.1023/A:1017994131769
Roza V. Menshova, Stanislav D. Anastyuk, Svetlana P. Ermakova, Natalia M. Shevchenko, Vladimir I. Isakov, Tatiana N. Zvyagintseva, Structure and anticancer activity in vitro of sulfated galactofucan from brown alga Alaria angusta. Carbohydrate Polymers. ,vol. 132, pp. 118- 125 ,(2015) , 10.1016/J.CARBPOL.2015.06.020
Pamlea N. Brady, Megan A. Macnaughtan, Evaluation of colorimetric assays for analyzing reductively methylated proteins: Biases and mechanistic insights. Analytical Biochemistry. ,vol. 491, pp. 43- 51 ,(2015) , 10.1016/J.AB.2015.08.027
M.S. Patankar, S Oehninger, T Barnett, R.L. Williams, G.F. Clark, A revised structure for fucoidan may explain some of its biological activities Journal of Biological Chemistry. ,vol. 268, pp. 21770- 21776 ,(1993) , 10.1016/S0021-9258(20)80609-7
Maria de Jesus Raposo, Alcina de Morais, Rui de Morais, Marine Polysaccharides from Algae with Potential Biomedical Applications Marine Drugs. ,vol. 13, pp. 2967- 3028 ,(2015) , 10.3390/MD13052967
Woo Kim, Joo Park, Jae Park, Doo Choi, Yong Park, Purification and Characterization of a Fucoidanase (FNase S) from a Marine Bacterium Sphingomonas paucimobilis PF-1. Marine Drugs. ,vol. 13, pp. 4398- 4417 ,(2015) , 10.3390/MD13074398
Zacharias. Dische, Landrum B. Shettles, A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination. Journal of Biological Chemistry. ,vol. 175, pp. 595- 603 ,(1948) , 10.1016/S0021-9258(18)57178-7
Michael Somogyi, NOTES ON SUGAR DETERMINATION Journal of Biological Chemistry. ,vol. 195, pp. 19- 23 ,(1926) , 10.1016/S0021-9258(18)84465-9