Metabolomics-oriented isolation and structure elucidation of 37 compounds including two anthocyanins from Arabidopsis thaliana.

摘要: In order to conduct metabolomic studies in a model plant for genome research, such as Arabidopsis thaliana (Arabidopsis), it is prerequisite obtain structural information the isolated metabolites from of interest. this study, we relatively non-targeted way, aiming at construction metabolite standards and chemotaxonomic comparison. Anthocyanins (5 7) called A8 A10 were their structures elucidated cyanidin 3-O-[2-O-(beta-D-xylopyranosyl)-6-O-(4-O-(beta-D-glucopyranosyl)-E-p-coumaroyl)-beta-D-glucopyranoside]-5-O-[6-O-(malonyl)-beta-D-glucopyranoside] 3-O-[2-O-(2-O-(E-sinapoyl)-beta-D-xylopyranosyl)-6-O-(4-O-(beta-D-glucopyranosyl)-E-p-coumaroyl)-beta-D-glucopyranoside]-5-O-[beta-D-glucopyranoside] analyses 1D NMR, 2D NMR ((1)H NOE, (13)C HMBC HMQC), HRFABMS, FT-ESI-MS GC-TOF-MS data. addition, 35 known compounds, including six anthocyanins, eight flavonols, one nucleoside, indole glucosinolate, four phenylpropanoids derivative, together with three indoles, carotenoid, apocarotenoid, galactolipids, two chlorophyll derivatives, steroid, hydrocarbon, dicarboxylic acids, also identified spectroscopic