The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response
作者： Roberto Nicolete , Daiane F. dos Santos , Lúcia H. Faccioli
DOI: 10.1016/J.INTIMP.2011.05.014
关键词:
摘要: Biodegradable micro/nanoparticles generated from PLGA have recently attracted attention due to their clinically proven biocompatibility, especially for immunization purposes. These polymeric particulate delivery systems are able present antigens and activate both humoral cellular responses. Many studies discussed the ideal size of these particles in contributing generation different types immune response. However, do not demonstrate effect micro or nanoparticles, without any encapsulated bioactive, on phagocytic cells after uptake process. In this context, aim study was analyze vitro inflammatory behavior J774 murine macrophages particles' uptake, since nano/microparticles per se can differently cells, using appropriate receptors, inducing distinct An o/w emulsion solvent extraction-evaporation method chosen prepare particles. We determined diameters, zeta potential morphology. Fluorescent by "macrophage-like" also analyzed. To evaluate profile nanoparticles' we conducted NF-κB translocation assay confocal microscopy pro-inflammatory cytokines production provoked
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M S Hayden, A P West, S Ghosh, NF-kappaB and the immune response. Oncogene. ,vol. 25, pp. 6758- 6780 ,(2006) , 10.1038/SJ.ONC.1209943
M. E. Christine Lutsiak, Deborah R. Robinson, Conrad Coester, Glen S. Kwon, John Samuel, Analysis of poly(D,L-lactic-co-glycolic acid) nanosphere uptake by human dendritic cells and macrophages in vitro. Pharmaceutical Research. ,vol. 19, pp. 1480- 1487 ,(2002) , 10.1023/A:1020452531828
Rainer H. Müller, Dörte Rühl, Martin Lück, Bernd.‐R. Paulke, Influence of fluorescent labelling of polystyrene particles on phagocytic uptake, surface hydrophobicity, and plasma protein adsorption. Pharmaceutical Research. ,vol. 14, pp. 18- 24 ,(1997) , 10.1023/A:1012043131081
Ying Men, Régine Audran, Claudio Thomasin, Gérard Eberl, Stéphane Demotz, Hans Peter Merkle, Bruno Gander, Giampietro Corradin, MHC class I- and class II-restricted processing and presentation of microencapsulated antigens Vaccine. ,vol. 17, pp. 1047- 1056 ,(1999) , 10.1016/S0264-410X(98)00321-1
A.M Carcaboso, R.M Hernández, M Igartua, J.E Rosas, M.E Patarroyo, J.L Pedraz, Potent, long lasting systemic antibody levels and mixed Th1/Th2 immune response after nasal immunization with malaria antigen loaded PLGA microparticles. Vaccine. ,vol. 22, pp. 1423- 1432 ,(2004) , 10.1016/J.VACCINE.2003.10.020
Daiane F dos Santos, Roberto Nicolete, Patrícia RM de Souza, Cláudia da S Bitencourt, Rubens R dos Santos Junior, Vânia LD Bonato, Célio L Silva, Lúcia H Faccioli, None, Characterization and in vitro activities of cell-free antigens from Histoplasma capsulatum-loaded biodegradable microspheres. European Journal of Pharmaceutical Sciences. ,vol. 38, pp. 548- 555 ,(2009) , 10.1016/J.EJPS.2009.10.003
Raghavendra C Mundargi, V Ramesh Babu, Vidhya Rangaswamy, Pradip Patel, Tejraj M Aminabhavi, None, Nano/micro technologies for delivering macromolecular therapeutics using poly(D,L-lactide-co-glycolide) and its derivatives. Journal of Controlled Release. ,vol. 125, pp. 193- 209 ,(2008) , 10.1016/J.JCONREL.2007.09.013
Roberto Nicolete, Karla de Melo Lima, José MR Júnior, Marcelo D Baruffi, Alexandra I de Medeiros, Maria VLB Bentley, Célio L Silva, Lúcia H Faccioli, None, In vitro and in vivo activities of leukotriene B4-loaded biodegradable microspheres. Prostaglandins & Other Lipid Mediators. ,vol. 83, pp. 121- 129 ,(2007) , 10.1016/J.PROSTAGLANDINS.2006.10.007
Yasuhiko Tabata, Yoshito Ikada, Macrophage phagocytosis of biodegradable microspheres composed of L-lactic acid/glycolic acid homo- and copolymers. Journal of Biomedical Materials Research. ,vol. 22, pp. 837- 858 ,(1988) , 10.1002/JBM.820221002
W JIANG, R GUPTA, M DESHPANDE, S SCHWENDEMAN, Biodegradable poly(lactic-co-glycolic acid) microparticles for injectable delivery of vaccine antigens Advanced Drug Delivery Reviews. ,vol. 57, pp. 391- 410 ,(2005) , 10.1016/J.ADDR.2004.09.003