Requirements for suppression of a dnaG mutation by an I-type plasmid.

摘要: Abstract We have confirmed and further characterized the phenomenon of suppression a dnaG mutation by an I plasmid determined requirements for complete expression suppressibility. The authentic derepressed conjugal fertility system, described earlier, was shown to be one requirements. second requirement is previously undescribed type leading far higher degree derepression this system than drd mutation. This leads extremely high pilus production. A third suppressibility that host recA function remain intact. required not merely fulfillment other two above, but also additional step or steps its expression. nature step(s) unknown, it insertion into genome.