Effects of 2,4-D and zeatin riboside on pollen callus induction in barley anther culture

摘要: Anthers of two barley (Hordeum vulgare L.) cultivars, Bonanza and Elrose were cultured in media A or K1 with different levels 2,4-D zeatin riboside (Z-r). The results indicated that a combination Z-r their optimal ranges (0.25–1.0 mg/L 0.125–0.25 mg/L, respectively) significantly increased pollen callus induction anthers. Addition alone (optimal range 0.25–2.0 mg/L) the frequency formation Elrose, while (0.125–2.0 mg/L) had no obvious effect on induction. When concentration was higher than 2.0 mg/L it decreased formation. At high concentrations (2.0–4.0 mg/L) induced loose calli which not able to develop into plantlets.Key words: Barley, anther culture, 2,4-D,