Overexpression of a type-I isopentenyl pyrophosphate isomerase of Artemisia annua in the cytosol leads to high arteannuin B production and artemisinin increase.

摘要: We recently characterized a gene-terpene network that is associated with artemisinin biosynthesis in self-pollinated (SP) Artemisia annua, an effective antimalarial plant. hypothesize alteration of gene expression the may improve production and its precursors. In this study, we cloned isopentenyl pyrophosphate isomerase (IPPI) cDNA, AaIPPI1, from annua (Aa). The full-length cDNA encodes type-I IPPI containing plastid transit peptide (PTP) at amino terminus. After removal PTP, recombinant truncated AaIPPI1 isomerized (IPP) to dimethyl allyl (DMAPP) vice versa. steady-state equilibrium ratio IPP/DMAPP enzymatic reactions was approximately 1:7. overexpressed cytosol SP A. annua variety. leaves transgenic plants produced 4% arteannuin B (g g-1 , dry weight, dw) 0.17-0.25% dw), levels which were significantly higher than those wild-type plants. addition, showed increase artemisinic acid more 1% dw). contrast, isoprene formation reduced These results provide evidence overexpression can lead metabolic alterations terpenoid biosynthesis, show these have potential yield high as new precursor source for artemisinin.