Effects of Maackia amurensis seed lectin (MASL) on oral squamous cell carcinoma (OSCC) gene expression and transcriptional signaling pathways.

摘要: Oral cancer causes over 120,000 deaths annually and affects the quality of life for survivors. Over 90% oral cancers are derived from squamous cell carcinoma cells (OSCCs) which generally resistant to standard cytotoxic chemotherapy agents. OSCC often exhibit increased TGFβ PDPN receptor activity compared nontransformed epithelial cells. Maackia amurensis seed lectin (MASL) can target has been identified as a novel agent that be used treat cancer. However, mechanisms by MASL inhibits progression not yet clearly defined. Here, we performed migration cytotoxicity assays assess effects on motility viability at physiologically relevant concentrations. We then comprehensive transcriptome analysis combined with transcription factor reporter investigate how gene expression these concentration. Key data were confirmed western blotting evaluate kinase signaling protein level. significantly affected about 27% approximately 15,000 genes found expressed HSC-2 model in this study. These include members TGFβ–SMAD, JAK–STAT, Wnt–βCTN pathways. In particular, decreased PDPN, SOX2, SMAD5 RNA levels. also inhibited SMAD MAPK activity, exhibited potential combination therapy doxorubicin 5-fluorouracil. Taken together, results study indicate decreases pathways inhibit growth motility. suggest further studies should undertaken determine may alone other agents