Comparative quantitation of abscisic acid in plant extracts by gas-liquid chromatography and an enzyme-linked immunosorbent assay using the avidin-biotin system.
作者： B. Leroux , R. Maldiney , E. Miginiac , L. Sossountzov , B. Sotta
DOI: 10.1007/BF00391277
关键词:
摘要: In this report we describe an enzyme-linked immunosorbent assay (ELISA) for the quantitation of abscisic acid (ABA) in plant extracts. A microtitration plate is coated with ABA-protein complex. The ABA, standard or sample, then added to each well a limiting quantity rabbit anti-ABA antibodies. During following incubation period, antibodies bind either free bound ABA on plates. After washing, are indirectly labelled two steps by means biotinylated goat antirabbit immunoglobulin-G which act as link between and avidin-alkaline phosphatase relative enzyme activity measured spectrophotometrically. detection limit method 5 pg measuring range extends 10 ng. Gas-liquid-chromatography controls, electron capture detector, show good correlation ELISA results obtained using extracts Lycopersicon esculentum, Nicotiana tabacum Pseudotsuga menziesii samples purified high-performance liquid chromatography. This provides argument accuracy immunoenzymatic method. indirect labelling antibodies, avidin-biotin amplifying system, should make technique suitable other growth substances against specific available.
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