Expression quantitative trait loci in long non-coding RNA ZNRD1-AS1 influence both HBV infection and hepatocellular carcinoma development

摘要: Zinc ribbon domain containing 1 (ZNRD1), cloned from human leukocyte antigen (HLA) region, may play integral roles in diverse processes including immune response against HBV infection and hepatocarcinogenesis. ZNRD1-AS1 (ZNRD1 antisense RNA 1) be an important regulator of ZNRD1. By bioinformatics analyses, we identified that several single nucleotide polymorphisms (SNPs) expression quantitative trait loci (eQTLs) for In this study, hypothesized these eQTLs SNPs influence both chronic hepatocellular carcinoma (HCC) development. We designed a case-control study 1300 HBV-positive HCC patients, 1344 persistent carriers and, natural clearance subjects to test the associations three ZNRD1 (rs3757328, rs6940552 rs9261204) with risk HCC. Logistic regression analyses additive genetic model showed variant alleles all increased host risk, whereas allele rs3757328 was associated clearance. Moreover, haplotype significantly development (adjusted OR = 1.18, 95% CI = 1.01-1.38, P = 0.035) OR = 0.83, CI = 0.71-0.96, P = 0.013), when compared most frequent haplotype. vitro experiments knockdown inhibited mRNA promoted proliferation HepG2.2.15 cells. These findings suggest regulatory susceptibility makers