Impairment of mitochondrial respiratory chain activity in aortic endothelial cells induced by glycated low-density lipoprotein
作者： Ganesh V. Sangle , Subir K. Roy Chowdhury , Xueping Xie , Gerald L. Stelmack , Andrew J. Halayko
DOI: 10.1016/J.FREERADBIOMED.2009.12.017
关键词:
摘要: Abstract Coronary artery disease (CAD) is the leading cause of mortality in diabetic patients. Mitochondrial dysfunction and increased production reactive oxygen species (ROS) are associated with diabetes CAD. Elevated levels glycated LDL (glyLDL) were detected patients diabetes. Our previous studies demonstrated that glyLDL generation ROS altered activities antioxidant enzymes vascular endothelial cells (EC). This study examined effects on consumption mitochondria key mitochondrial electron transport chain (ETC) cultured porcine aortic EC. The results treatment significantly impaired Complexes I, II/III, IV ETC EC compared to or vehicle control using oxygraphy. Incubation reduced membrane potential, NAD + /NADH ratio, (NADH-ubiquinone dehydrogenase, succinate cytochrome c reductase, ubiquinone oxidase) control. abundance mitochondria-associated release from after treatment. findings suggest attenuates ETC, decreases consumption, reduces increases EC, which potentially contribute
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sci-hub.se 参考文章(67)
David C. Wharton, Alexander Tzagoloff, [45] Cytochrome oxidase from beef heart mitochondria Methods in Enzymology. ,vol. 10, pp. 245- 250 ,(1967) , 10.1016/0076-6879(67)10048-7
Hani Atamna, Jiankang Liu, Bruce N. Ames, Heme Deficiency Selectively Interrupts Assembly of Mitochondrial Complex IV in Human Fibroblasts RELEVANCE TO AGING Journal of Biological Chemistry. ,vol. 276, pp. 48410- 48416 ,(2001) , 10.1074/JBC.M108362200
Takeshi Nishikawa, Diane Edelstein, Xue Liang Du, Sho-ichi Yamagishi, Takeshi Matsumura, Yasufumi Kaneda, Mark A. Yorek, David Beebe, Peter J. Oates, Hans-Peter Hammes, Ida Giardino, Michael Brownlee, Normalizing mitochondrial superoxide production blocks three pathways of hyperglycaemic damage Nature. ,vol. 404, pp. 787- 790 ,(2000) , 10.1038/35008121
Shigeru Hokari, Ikuo Inoue, Shin-ichiro Kawai, Iwao Koyama, Kazuhiro Iguchi, Kazuyuki Hirano, Takanori Nakajima, Takashi Miyazaki, Toshiyuki Matsunaga, Tsugikazu Komoda, Shigehiro Katayama, Glycated High-Density Lipoprotein Induces Apoptosis of Endothelial Cells via Mitochondrial Dysfunction. The annual proceedings of Gifu College of Pharmacy. ,vol. 51, pp. 65- ,(2002)
Holly R. McLennan, Mauro Degli Esposti, The contribution of mitochondrial respiratory complexes to the production of reactive oxygen species. Journal of Bioenergetics and Biomembranes. ,vol. 32, pp. 153- 162 ,(2000) , 10.1023/A:1005507913372
Daniel Floryk, Josef Houštêk, Tetramethyl Rhodamine Methyl Ester (TMRM) is Suitable for Cytofluorometric Measurements of Mitochondrial Membrane Potential in Cells Treated with Digitonin Bioscience Reports. ,vol. 19, pp. 27- 34 ,(1999) , 10.1023/A:1020193906974
Anabela P. Rolo, Carlos M. Palmeira, Diabetes and mitochondrial function: role of hyperglycemia and oxidative stress. Toxicology and Applied Pharmacology. ,vol. 212, pp. 167- 178 ,(2006) , 10.1016/J.TAAP.2006.01.003
Ruozhi Zhao, Garry X. Shen, Functional modulation of antioxidant enzymes in vascular endothelial cells by glycated LDL Atherosclerosis. ,vol. 179, pp. 277- 284 ,(2005) , 10.1016/J.ATHEROSCLEROSIS.2004.11.013
Hui Wang, Jianghai Liu, Lingyun Wu, Methylglyoxal-induced mitochondrial dysfunction in vascular smooth muscle cells Biochemical Pharmacology. ,vol. 77, pp. 1709- 1716 ,(2009) , 10.1016/J.BCP.2009.02.024
Enzo Nisoli, Emilio Clementi, Michele O. Carruba, Salvador Moncada, Defective Mitochondrial Biogenesis: A Hallmark of the High Cardiovascular Risk in the Metabolic Syndrome? Circulation Research. ,vol. 100, pp. 795- 806 ,(2007) , 10.1161/01.RES.0000259591.97107.6C