Fluorescence lifetime spectroscopy for breast cancer margins assessment

摘要: During breast conserving surgery (BCS), which is the preferred approach to treat most early stage cancers, surgeon attempts excise tumor volume, surrounded by thin margin of normal tissue. The intra-operative assessment cancerous areas a challenging procedure, with surgeon usually relying on visual or tactile guidance. This study evaluates whether time-resolved fluorescence spectroscopy (TRFS) presents potential address this problem. Point TRFS measurements were obtained from 19 fresh tissue slices (7 patients) and parameters that characterize transient signals quantified via constrained least squares deconvolution scheme. Fibrotic (FT, n=69), adipose (AT, n=76), invasive ductal carcinoma (IDC, n=27) identified in histology univariate statistical analysis, followed multi-comparison test, was applied corresponding lifetime data. Significant differentiation between three types exists at 390 nm 500 bands. average 3.23±0.74 ns for AT, 4.21±0.83 FT 4.71±0.35 (p<0.05) IDC nm. Due smaller contribution of collagen AT value different IDC. Additionally, although intensity measurements do not show difference IDC, can distinguish them. Similarly, these values are 7.01±1.08 ns, 5.43±1.05 4.39±0.88 correspondingly contrast due differentiation retinol flavins relative concentration, mostly contributing AT. Results demonstrate TRFS intra-operatively characterize BCS excised real-time assess margins.