Effect of tiotropium bromide on airway inflammation and remodelling in a mouse model of asthma

摘要: Summary Background Tiotropium bromide, a long acting muscarinic receptor inhibitor, is potent agent for patients with bronchial asthma as well chronic obstructive pulmonary disease. Objective The aim of this study was to evaluate whether tiotropium bromide can inhibit allergen-induced acute and airway inflammation, T helper (Th)2 cytokine production, remodelling in murine model asthma. Methods Balb/c mice were sensitized challenged acutely or chronically ovalbumin (OVA). impact assessed using these models by histologic, morphometric, molecular techniques. Moreover, the effect on Th2 production from purified human peripheral blood mononuclear cells (PBMCs) assessed. Results Treatment significantly reduced inflammation bronchoalveolar lavage fluid (BALF) both asthma. levels TGF-β1 also BALF model. goblet cell metaplasia, thickness smooth muscle, fibrosis all decreased bromide-treated mice. hyperresponsiveness (AHR) serotonin abrogated spleen isolated OVA-sensitized inhibited 4-diphenylacetoxy-N-methylpiperidine methiodide, which selective antagonist M3 receptor. Finally, treatment PBMCs. Conclusion These results indicate that thus may reduce AHR asthma. Cite as: S. Ohta, N. Oda, T. Yokoe, A. Tanaka, Y. Yamamoto, Watanabe, K. Minoguchi, Ohnishi, Hirose, H. Nagase, Ohta M. Adachi, Clinical & Experimental Allergy, 2010 (40) 1266–1275.