The Helicase Activity of Hyperthermophilic Archaeal MCM is Enhanced at High Temperatures by Lysine Methylation
作者： Yisui Xia , Yanling Niu , Jiamin Cui , Yang Fu , Xiaojiang S. Chen
关键词:
摘要: Lysine methylation and methyltransferases are widespread in the third domain of life, archaea. Nevertheless, effects on archaeal proteins wait to be defined. Here, we report that recombinant sisMCM, an homolog Mcm2-7 eukaryotic replicative helicase, is methylated by aKMT4 vitro. Mono-methylation these lysine residues occurs coincidently endogenous sisMCM protein purified from hyperthermophilic Sulfolobus islandicus cells as indicated mass spectra. The helicase activity MCM stimulated methylation, particularly at temperatures over 70°C. shows optimal DNA unwinding after heat-treatment between 76 82°C, which correlates well with typical growth Sulfolobus. After half life dramatically extended 80°C. sites located accessible surface, might modulate intra- inter- molecular interactions through changing hydrophobicity surface charge. Furthermore, methylation-mimic mutants show heat resistance comparable MCM. These data provide biochemical evidence posttranslational modifications such may enhance kinetic stability under elevated
frontiersin.org
core.ac.uk
sci-hub.se 参考文章(52)
Ian M. Slaymaker, Xiaojiang S. Chen, MCM structure and mechanics: what we have learned from archaeal MCM. Sub-cellular biochemistry. ,vol. 62, pp. 89- 111 ,(2012) , 10.1007/978-94-007-4572-8_6
Xi Zhang, Yaling Huang, Xiaobing Shi, Emerging roles of lysine methylation on non-histone proteins Cellular and Molecular Life Sciences. ,vol. 72, pp. 4257- 4272 ,(2015) , 10.1007/S00018-015-2001-4
Marc R. Wilkins, Elisabeth Gasteiger, Amos Bairoch, Jean-Charles Sanchez, Keith L. Williams, Ron D. Appel, Denis F. Hochstrasser, Protein identification and analysis tools in the ExPASy server Methods of Molecular Biology. ,vol. 112, pp. 531- 552 ,(1999) , 10.1385/1-59259-584-7:531
Ian M. Slaymaker, Yang Fu, Daniel B. Toso, Nimna Ranatunga, Aaron Brewster, Susan L. Forsburg, Z. Hong Zhou, Xiaojiang S. Chen, Mini-chromosome maintenance complexes form a filament to remodel DNA structure and topology Nucleic Acids Research. ,vol. 41, pp. 3446- 3456 ,(2013) , 10.1093/NAR/GKT022
Matthew L. Bochman, Anthony Schwacha, The Mcm2-7 complex has in vitro helicase activity. Molecular Cell. ,vol. 31, pp. 287- 293 ,(2008) , 10.1016/J.MOLCEL.2008.05.020
Thomas R Beattie, Stephen D Bell, Molecular machines in archaeal DNA replication Current Opinion in Chemical Biology. ,vol. 15, pp. 614- 619 ,(2011) , 10.1016/J.CBPA.2011.07.017
Steven G. Clarke, Protein methylation at the surface and buried deep: thinking outside the histone box Trends in Biochemical Sciences. ,vol. 38, pp. 243- 252 ,(2013) , 10.1016/J.TIBS.2013.02.004
Matthew J. Moreau, Adam T. McGeoch, Alan R. Lowe, Laura S. Itzhaki, Stephen D. Bell, ATPase Site Architecture and Helicase Mechanism of an Archaeal MCM Molecular Cell. ,vol. 28, pp. 304- 314 ,(2007) , 10.1016/J.MOLCEL.2007.08.013
Cyrus Martin, Yi Zhang, The diverse functions of histone lysine methylation. Nature Reviews Molecular Cell Biology. ,vol. 6, pp. 838- 849 ,(2005) , 10.1038/NRM1761
E. R. Barry, J. E. Lovett, A. Costa, S. M. Lea, S. D. Bell, Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 106, pp. 1051- 1056 ,(2009) , 10.1073/PNAS.0809192106