A method for continuously assessing coronary blood flow velocity in the rat

摘要: We have developed a directional pulsed-Doppler system to make blood flow velocity measurements in the coronary arteries of rat. The probe consists 1-mm2 crystal mounted 6-mm suction cup, which can be attached by vacuum vessel without requiring dissection. Recordings phasic (CBV) rat indicate that 82 +/- 2% (mean SE) area under CBV recording occurs diastole. increased during an infusion dipyridamole and changed parallel with alterations left-ventricular pressure. To validate technique we correlated changes wih microsphere-measured perfusion (range, 20—;780 ml/min x 100 g). These two methods estimating closely (r = 0.93). Measurements this Doppler should permit detailed characterization circulation many models disease been