Production of platelet-activating factor by chick retina.

摘要: In the present study it is demonstrated that platelet-activating factor (PAF) was produced by chick retinas, upon stimulation with neurotransmitters such as acetylcholine (ACh), dopamine, or calcium ionophore A23187, but not gamma-amino-n-butyric acid, L-glycine, L-glutamate, epinephrine, histamine. PAF in response to ACh, A23187 released into supernatants extractable from retinas. The amounts of increased after sonication stimulated While no activity could be recovered unstimulated small this lipid can detected following tissue. amount ACh-, dopamine-, A23187-stimulated retinas dependent incubation time and concentration agonists. identified on basis chemical lipase treatments, biological washed rabbit platelets, behavior thin layer chromatography, high pressure liquid chromatography. Control cell preparations (leukocytes, erythrocytes, embryogenic fibroblasts) did produce neurotransmitter stimulation. ACh dopamine promoted production increasing dithiothreitol-insensitive cholinephosphotransferase activity, without affecting acetyltransferase activity. contrast, affect cholinephosphotransferase.