Ontogeny of voltage-sensitive calcium channel α1A and α1E subunit expression and synaptic function in rat central nervous system

摘要: Abstract Immunohistochemical expression in the neocortex, hippocampus and cerebellum of α1A or α1E subunit voltage-sensitive Ca2+ channel was examined Long–Evans hooded rats on gestational day 18 postnatal days 1, 4, 7, 10, 14, 21, 90, 360 720. On immunoreactivity more dense neocortex than cerebellum. By levels increased dramatically cerebellum, while became sparse, which approached diffuse pattern cellular staining mature brain. Expression much less between 4. There also significant mossy fibers dendrites Purkinje cells Depolarization-dependent 45Ca2+ influx rat brain synaptosomes 21 >60. In neocortical hippocampal synaptosomes, steadily with age reached adult by 10. cerebellar constant across all ages, except for a spike activity observed 21. 100 nM ω-conotoxin MVIIC significantly inhibited 10 respectively, after. only prior. not combination μM nifedipine, 1 GVIA MVIIC, suggesting that an ‘insensitive’ flux predominates at this age. Overall, results suggest channels during development is dynamic important central nervous system development.