Delayed Engraftment of Nonobese Diabetic/Severe Combined Immunodeficient Mice Transplanted With Ex Vivo–Expanded Human CD34+ Cord Blood Cells

摘要: The ex vivo expansion of hematopoietic progenitors is a promising approach for accelerating the engraftment recipients, particularly when cord blood (CB) used as source graft. With aim defining in repopulating properties vivo-expanded CB cells, purified CD34(+) cells were subjected to expansion, and equivalent proportions fresh samples transplanted into irradiated nonobese diabetic (NOD)/severe combined immunodeficient (SCID) mice. At periodic intervals after transplantation, femoral bone marrow (BM) obtained from NOD/SCID recipients kinetics evaluated individually. transplantation generated dose-dependent which was evident all posttransplantation times analyzed (15 120 days). When compared with CB, stimulated 6 days interleukin-3 (IL-3)/IL-6/stem cell factor (SCF) contained increased numbers (20-fold increase colony-forming unit granulocyte-macrophage [CFU-GM]). However, significant impairment short-term repopulation associated versus (CD45(+) NOD/SCIDs BM: 3. 7% +/- 1.2% v 26.2% 5.9%, respectively, at 20 posttransplantation; P <.005). An impaired also observed mice incubated IL-11/SCF/FLT-3 ligand (3.5% 1.7% CD45(+) BM posttransplantation). In contrast these data, similar later stages posttransplantation. days, CD45(+)/CD34(+) ranged between 67.2% 81.1% 8.6% 12.6%, no differences found. analysis engrafted showed that both vitro-incubated capable lymphomyeloid reconstitution. Our results suggest although preserves long-term ability sample, an unexpected delay manipulated cells.