Influence of solid surface, adhesive ability, and inoculum size on bacterial colonization in microcosm studies.

摘要: Microcosm studies were performed to evaluate the effect of solid surfaces, bacterial adhesive ability, and inoculum size on colonization success persistence Pseudomonas fluorescens Xanthomonas maltophilia, each with a Tn5 insertion that conferred resistance kanamycin streptomycin. Two types microcosms used: (i) simple system was colonized by Aeromonas hydrophila coryneform (ii) complex produced from lake water enrichment cultures. Simple contained 100 ml peptone- yeast extract-supplemented artificial or 60 70 g 3-mm glass beads. Complex no nutrient additions The incubated for 35 days at 20°C. In microcosms, presence beads increased abilities P. X. maltophilia colonize, but their numbers decreased time in both without adhesiveness bacteria, measured an vitro assay, did not relate success. (10, 102, 103) influence However, 103 cells insufficient ensure fluorescens, while 106 resulted liquid microcosm studies, utilizing only few species, poor models natural systems. systems, surfaces higher organisms noticeably promote persistence. Adhesiveness particular organism may be relatively minor factor influencing its ability colonize environments.