Rapid characterization the chemical constituents of Bergenia purpurascens and explore potential mechanism in treating osteoarthritis by ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry combined with network pharmacology.

摘要: In recent years, direct and indirect evidence has been found of the efficacy traditional Chinese medicine Bergenia purpurascens in treating arthritis osteoarthritis. Several major components, such as bergenin 11-O-galloylbergenin, have good anti-inflammatory activity. Since research on chemical components related mechanisms for treatment osteoarthritis never performed, this study aimed to analyze through ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry technology UNIFI screening platform predict underlying by analyzing network pharmacology. total, 43 constituents were identified, mainly flavonoids (18), phenolic glycosides (13), organic acids (7). Among them, 16 first time. Through analysis pharmacology, several potential candidate targets pathways initially predicted, including AKT1, MAPK1, MAPK3, well apoptosis, estrogen, MAPK signaling pathways. Bergenin, arbutin, catechin-3-O-gallate, other play a synergistic role This analyzed preliminarily revealed osteoarthritis, providing basis further evaluating drug's efficacy.