Simple quantification of phenolic compounds present in the minor fraction of virgin olive oil by LC-DAD-FLD.

摘要: Abstract This paper presents the results of study on extraction, identification and quantification a group important phenolic compounds in virgin olive oil (VOO) samples, obtained from olives various varieties, by liquid chromatography coupled to UV–vis fluorescence detection. Sixteen belonging different families have been identified quantified spending total time 25 min. The linearity was examined establishing external standard calibration curves. Four order linear ranges limits detection ranging 0.02 0.6 μg mL −1 0.006 0.3 μg mL were achieved using detection, respectively. Regarding real for determination higher concentrations (hydroxytyrosol tyrosol) simple liquid–liquid extraction with ethanol used make sample compatible mobile phase. Recovery values close 100% obtained. However, previous solid phase Diol cartridges necessary concentrate separate minor main interferences. parameters affecting this step carefully optimized and, after that, recoveries near 80–100% rest studied compounds. Also, improved 15 times. Finally, addition method carried out each analytes no matrix effect found, so 16 monovarietal VOO corresponding plot.