Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging

摘要: Living cells are chemical mixtures of exceptional interest and significance, whose investigation requires the development powerful analytical tools fulfilling demanding constraints resulting from their singular features. In particular, multiplexed observation a large number molecular targets with high spatiotemporal resolution appears highly desirable. One attractive road to address this challenge relies on engaging in reactions exploiting rich kinetic signature reactive module, which originates its topology rate constants. This review explores various facets promising strategy. We first emphasize singularity content living cell as mixture suggest that is significant timely. Then, we show kinetics processes relevant selectively detect given analyte: upon perturbing system, window associated response read-out has be matched targeted module. Eventually, introduce state-of-the-art imaging protocols based reaction draw some perspectives.