The Role of NADPH Oxidase in the Inhibition of Trichophyton rubrum by 420-nm Intense Pulsed Light.
作者： Hao Huang , Weibiao Lv , Ying Chen , Xiufeng Zheng , Yong Hu
关键词:
摘要: Objectives: To evaluate the effect of intense pulsed light (IPL) on Trichophyton rubrum and investigate its mechanism action. Methods: The viability fungi treated with IPL alone combined an NADPH oxidase inhibitor (DPI) pretreatment was determined by MTT assays. reactive oxygen species (ROS) were quantified a DCFH-DA fluorescent probe. Malondialdehyde (MDA) content superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) activities commercial kits. transcription Nox gene using quantitative real-time PCR (qRT-PCR) analysis, micromorphology observed scanning electron microscopy (SEM). In addition, fungal keratinase activity detected measuring dye release from keratin azure. Results: growth declined statistical significance after 6 h treatment (P < 0.001). ROS MDA increased treatment, whereas SOD GSH-Px decreased. expression upregulated, damaged. Keratinase Fungi that received DPI exhibited contrasting outcomes. Conclusion: We found 420-nm significantly inhibited pathogenicity T. in vitro. A suggested involves as factor mediates IPL-induced oxidative damage rubrum.
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sci-hub.se 参考文章(57)
Amy Forman Taub, A comparison of intense pulsed light, combination radiofrequency and intense pulsed light, and blue light in photodynamic therapy for acne vulgaris. Journal of Drugs in Dermatology. ,vol. 6, pp. 1010- 1016 ,(2007)
Monika Schäfer-Korting, [New systemic antifungal drugs: mechanisms of action, drug interactions and side effects]. Mycoses. ,vol. 1, pp. 28- 31 ,(2003)
Seyed Alireza Ghavam, Hamideh Moravvej, Ezedin Mohajerani, Saeed Aref, Mohammad Reza Shidfar, LASER IRRADIATION ON GROWTH OF TRICHOPHYTON RUBRUM: AN IN VITRO STUDY Journal of lasers in medical sciences. ,vol. 6, pp. 10- 16 ,(2015) , 10.22037/2010.V6I1.6454
T P A Devasagayam, Saroj S Ghaskadbi, Ketaki S Sane, K K Boloor, R D Lele, J C Tilak, Free radicals and antioxidants in human health: current status and future prospects. Journal of Association of Physicians of India. ,vol. 52, pp. 794- 804 ,(2004)
Pietro Nenoff, Constanze Krüger, Gabriele Ginter-Hanselmayer, Hans-Jürgen Tietz, Mycology – an update. Part 1: Dermatomycoses: Causative agents, epidemiology and pathogenesis Journal Der Deutschen Dermatologischen Gesellschaft. ,vol. 12, pp. 188- 210 ,(2014) , 10.1111/DDG.12245
Chi K. Yeung, Samantha Y. Shek, Peter Bjerring, Carol S. Yu, Taro Kono, Henry H. Chan, A comparative study of intense pulsed light alone and its combination with photodynamic therapy for the treatment of facial acne in Asian skin. Lasers in Surgery and Medicine. ,vol. 39, pp. 1- 6 ,(2007) , 10.1002/LSM.20469
DevinderMohan Thappa, Current treatment of onychomycosis. Indian Journal of Dermatology, Venereology and Leprology. ,vol. 73, pp. 373- 376 ,(2007) , 10.4103/0378-6323.37052
Chia-Jui Weng, Mei-Jung Chen, Chi-Tai Yeh, Gow-Chin Yen, Hepatoprotection of quercetin against oxidative stress by induction of metallothionein expression through activating MAPK and PI3K pathways and enhancing Nrf2 DNA-binding activity. New Biotechnology. ,vol. 28, pp. 767- 777 ,(2011) , 10.1016/J.NBT.2011.05.003
Nihar R Biswas, Hrishikesh Das, Gita Satpathy, Srujana Mohanty, Anita Panda, None, Role of Aprotinin in the Management of Experimental Fungal Keratitis Ophthalmic Research. ,vol. 33, pp. 147- 150 ,(2001) , 10.1159/000055661
A.J. Samdani, P.J. Dykes, R. Marks, The proteolytic activity of strains of T. mentagrophytes and T. rubrum isolated from tinea pedis and tinea unguium infections Medical Mycology. ,vol. 33, pp. 167- 170 ,(1995) , 10.1080/02681219580000351