Angiogenic switch occurs during the precancerous stage of human esophageal squamous cell carcinoma

摘要: We previously reported that vascular endothelial growth factor (VEGF) expression correlates with vessel density in human esophageal squamous cell carcinomas. However, tumor angiogenesis is not controlled simply by the presence of VEGF, and likely regulated several angiogenic factors produced host cells. The goal present study was to determine profile precancerous cancerous lesions esophagus. Expression mRNAs for platelet derived (PD-ECGF), basic fibroblast (bFGF), interleukin (IL)-8 examined six carcinoma lines fresh biopsy specimens from 16 patients invasive RT-PCR. Immunohistochemical analyses antibodies against PD-ECGF, bFGF, IL-8 were performed on archival 60 normal mucosa, 11 dysplasias 49 carcinomas Microvessels stained anti-CD34 antibody quantified counting number vessels a x200 field most vascularized areas tumor. Esophageal tissues expressed one or more these at various levels. An initial increase enhanced PD-ECGF VEGF observed dysplastic epithelium. Vessel significantly higher advanced lesions. bFGF mucosal carcinomas, but increased late stage carcinoma. These findings suggest switch very early event development Several different cells may regulate during steps carcinogenesis.