Studies on the mechanism of embryonic frog nerve orientation in a small applied electric field

摘要: The mechanism of nerve orientation in an applied electric field has been investigated using a number pharmacological agents. Galvanotropism may depend on redistribution within the plasma membrane integral proteins (IMP); blocking this with concanavalin A inhibited orientation. Orientation also influx Ca2+; Co2+ and La3+ blockade calcium channels turning field. Organic blockers did not influence orientation, suggesting that L-type Ca2+ be present at growth cone. Procedures induce asymmetric entry anodal side cells caused reversal normal galvanotropism, directed towards anode. This implicate local levels cytoplasmic cone controlling behaviour. An distribution filopodia precedes predict direction Various agents perturbed such way reflect subsequent It is suggested that, normally, increases asymmetry operate together determining but filopodial activity subordinate to can overriden by In addition, two drug treatments markedly increased rates growth, which importance regeneration.