Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium.

摘要: The luminal and basal epithelial cells in the human mammary gland can be distinguished tissue sections on basis of pattern keratins they express. Moreover, invasive primary carcinomas show a keratin profile that corresponds to dominant cell (7, 8, 18, 19). When homogeneous populations from milk or breast cancer metastases are cultured expression seen vivo is maintained. We have therefore used monospecific antibodies reactive with individual examine phenotype three different media reduction mammoplasty contains both cells. cancers one these (MCDB170) has also been examined. In characterizing phenotypes, polymorphic mucin (PEM) expressed by cells, smooth muscle (a) actin, used. Our results proliferation phenotypes selected for media. mix (MX) developed growth milk, only proliferates (for 1 2 passages). medium MCDB 170, which was long-term organoids, layer proliferate, while MM dominates, but few found. Around passage 3, most senesce subpopulation further passage. These retain express some features characteristic suggesting may contain stem develop along lineage. culture, however, do not 19, feature fully differentiated cell. 170 similar normal this medium. They even though homogeneously vivo. phenotype, its metastatic lines, cannot using MX, supports corresponding